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Super-res of septin bundles - a collaboration between the Yip and Trimble labs

Congrats to Adriano on his collaborative paper with the Trimble lab on super-resolution imaging of septin bundles

 

https://www.ncbi.nlm.nih.gov/pubmed/30176126?dopt=Abstract

 

Adriano used both dSTORM and PALM : We report here on a Direct Stochastic Optical Reconstruction Microscopy (dSTORM) and Photoactivated Localization Microscopy (PALM) study of the 2D spatial distribution of septins in mammalian cells. From simulated and experimental results, we show the effects of labeling method, labeling efficiency, and fluorescent emitter photophysics on image reconstruction and interpretation. Our experimental results are consistent with septin organization by polymerization of hetero-octamers and an approximate 30-35 nm periodicity between subsequent units of SEPT2-SEPT2 or SEPT9-SEPT9.

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